ADCC / Fc gamma Receptor BINDING ASSAYS

Fc gamma receptors are a family of glycoproteins expressed on a variety of immune cells with affinity for the Fc region of immunoglobulin G (IgG). The receptor family includes several members: FcγRI (CD64), FcγRIIa/b/c (CD32a/b/c), and FcγRIIIa/b (CD16a/b) with different polymorphic variants. Each of them has characteristic preferences for the binding of IgG isotypes and can transduce either activating (e.g. FcγRI, IIa, IIIa) or inhibitory (e.g. FcγRIIb) signals.  FcγRs have been shown to be important in modulating the efficacy of therapeutic mAbs by FcγR-mediated phagocytosis, cytokine production, antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cell-mediated phagocytosis (ADCP). The affinity and effector function is critically effected by the N-glycosylation profile of FcγR and antibody.

The neonatal Fc receptor (FcRn) is structurally distinct and binds to IgG within the acidic environment (pH < 6.5) of endosomes but, in contrast, not at physiological pH. FcRn enables transcytosis of IgG and mediates the half-life of IgG and therapeutic antibodies.

Biaffin provides FcR binding assay using Biacore T200 instruments for getting detailed kinetic information about antibody - receptor interaction. Two different assay setups are established in opposing coupling orientations to choose the ideally suited assay for your research.