QUANTITATIVE kinetic characterisation
Dou you need a comprehensive kinetic characterisation of your antibodies? Our BIA Antibody Service is able to accurately characterize binding kinetics and affinities of your antibodies by SPR, the gold standard of label-free interaction analysis.
A quantitative analysis includes a detailed kinetic characterisation of antigen - antibody interaction with an exact determination of binding affinity and kinetic rate constants.
Our quantitative antibody service includes either the fast STANDARD antibody characterisation or a detailed, more individual PREMIUM analysis of the binding event.
Our STANDARD ANTIBODY service:
- coupling of the antibody to a specific biosensor capture surface
- analysis of the antibody/antigen interaction by a complete dilution series of the antigen
- regeneration of the capture surface after each interaction and new coupling of antibody
- standard analysis conditions (5 min association and dissociation time, standard buffer)
- evaluation based on a 1:1 Langmuir model
- basic report including calculation of affinities (KD-value) and rate constants for association (kass) and dissociation (kdiss) with figures of the dilution series
Our individual PREMIUM ANTIBODY service includes:
- additionally an optimisation of the experimental conditions (avoidance of unspecific binding, variation of buffer composition, individually adapted association and dissociation times as well as customized dilution series of the antigen)
- extended data evaluation (determination of kinetic constants KD-value, kass and kdiss by usage of different kinetic models
- detailed report including figures and data tables as well as a short discussion of the experimental method optimisation and the applied kinetic models
© 2024 | Biaffin GmbH & Co. KG
BIAFFIN GmbH & Co KG | Heinrich-Plett-Str. 40 | D-34132 Kassel | Germany
Tel.: +49 (0) 561-804 4661 | Fax: +49 (0) 561-804 4665 | info@biaffin.de
Tel.: +49 (0) 561-804 4661 | Fax: +49 (0) 561-804 4665 | info@biaffin.de
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