PROCESS OPTIMISATION

Example Affinity Chromatography:

Optimise binding and elution conditions (pH, ionic strength, wash steps, unspecific binding)

• Test novel affinity matrices
• Test for steric hindrance

Example: Influence of low molecular ligands on binding to the affinity matrix

Buffer composition strongly influences association and dissociation of the protein to be purified on the affinity matrix: addition of ligand can increase the capacity of the matrix and optimise elution conditions.

Take advantage of our experience in biomolecular interaction analysis. Please contact us for an individual quote for your specific question.

NUCLEIC ACIDS INTERACTIONS

BIA technology - an ideal tool for analysing nucleid acid interactions
BIA technology is ideally suited as a tool for analysing nucleic acid interactions like hybridization of single strand DNA or RNA molecules, binding of transcription factors to specific DNA sequences or the binding of RNA aptamers to protein targets. The label free and real time monitoring provide insight not only into specificity and affinity but also into the kinetics and complex mechanisms underlying these interactions.
Biotinylated nucleic acids can easily be used for immobilisation on streptavidin coated biochips. The generated DNA or RNA chips are useful for hybridisation experiments with complementary strands of modified or unmodified nucleic acids allowing analysis of the kinetic binding patterns depending on the nucleotide sequence, inserted mutations or deletions as well as buffer conditions (ionic strength, metal ions, etc.) and temperature. Another topic is the interaction of nucleic acids with proteins. BIA technology can help to understand the binding mechanisms and discover the binding motives of nucleic acid binding proteins like transcription factors.
Please contact us for more information or if you like to discuss future projects that we might perform for you. We will be glad to develop an adequate strategy that is especially suited to achieve your aims.

DNA-DNA

Hybridisation experiments with nucleic acids include in general

• the effect of hybridisation temperature
• the length of the nucleic acid sequence to hybridise
• the effect of base pair substitutions of the template

Biotinylated single or double stranded DNA or RNA can be immobilised highly reproducible in a one step procedure to streptavidin biochips. With this nucleic acid chips not only hybridisation experiments with natural nucleic acids can be performed but also with modified artificial nucleic acids derivates (like PNA - peptide nucleic acid). Alternatively binding studies with proteins (like transcription factors) can be performed.
BIA chip technology with nucleic acids allows a fast screening of PCR amplifications of genomic sequences. Binding studies of certain complexes of nucleic acids in HIV (on DNA and RNA level) are possible.
Further more specific detection of single stranded nucleic acids in complex serum samples can be performed. Biaffin provides a method for detection of DNA oligo nucleotides from human serum samples, which even allows quantification of unknown DNA content in serum after proteinase K digestion.