Fc gamma Receptor BINDING ASSAYS

Detailed kinetic analysis of FcγR and FcRn binding to antibodies using SPR-based assays

Fc gamma receptors are a family of glycoproteins expressed on a variety of immune cells with affinity for the Fc region of immunoglobulin G (IgG). The receptor family includes several members: FcγRI (CD64), FcγRIIa/b/c (CD32a/b/c), and FcγRIIIa/b (CD16a/b) with different polymorphic variants. Each of them has characteristic preferences for the binding of IgG isotypes and can transduce either activating (e.g. FcγRI, IIa, IIIa) or inhibitory (e.g. FcγRIIb) signals.  FcγRs have been shown to be important in modulating the efficacy of therapeutic mAbs by FcγR-mediated phagocytosis, cytokine production, antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cell-mediated phagocytosis (ADCP). The affinity and effector function is critically effected by the N-glycosylation profile of FcγR and antibody.

The neonatal Fc receptor (FcRn) is structurally distinct and binds to IgG within the acidic environment (pH < 6.5) of endosomes but, in contrast, not at physiological pH. FcRn enables transcytosis of IgG and mediates the half-life of IgG and therapeutic antibodies.

Biaffin provides FcR binding assay using Biacore T200 instruments for getting detailed kinetic information about antibody - receptor interaction. Two different assay setups are established in opposing coupling orientations to choose the ideally suited assay for your research.

 

 

CD64 Fc gamma RI SPR interaction analysis

Figure: Real-time kinetic analysis of FcγRI binding to antibody using surface plasmon resonance.

neonatal FcRn receptor SPR interaction analysis

Figure: Real-time kinetic analysis of antibody binding to FcRn using surface plasmon resonance.

Our service:

  • capture of antibody or Fc receptor using suitable coupling strategies
  • qualitative and quantitative kinetic analysis of FcγR/FcRn interaction providing rate constants for association and dissociation and affinities (ka, kd, KD)

If you are interested in performing a SPR study with us and getting more information and a work plan and quotation, please get in contact with one of our SPR application specialists.

BIAFFIN GmbH & Co KG | Heinrich-Plett-Str. 40 | D-34132 Kassel | Germany
Tel.: +49 (0) 561-804 4661 | Fax: +49 (0) 561-804 4665 | info@biaffin.de