Classical methods for characterisation of antigen - antibody interactions like ELISA only provide equilibrium binding data at one defined time point not considering rates of complex formation and complex dissociation.
SPR Biacore technology has been proven to be an excellent method to validate antibodies by monitoring binding kinetics between antigen and antibody in real time. The obtained binding curves contain detailed kinetic information about association and dissociation rates, about stoichiometry of binding and about binding activity and quality of samples. Furthermore, an assessment of binding characteristics regarding specificity, heterogeneity and avidity effects is feasible as well. Mode of action and temperature dependency of an interaction can be examined by analysing antibody antigen interactions at varying temperatures.
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