BIACORE 2000

The Biacore 2000 is a fully automated system ideal for assay development and fast and accurate routine applications like kinetic interaction analyses and concentration measurements.

The microfluidic system consists of four flow cells, arranged in a serial configuration for single or multi-channel analysis. The different flow cells can be prepared by covering different concentrations of the same ligand or comparing simultaneously three different ligands interacting with one analyte whereas one channel is used as in-line control cell.

The molecular detection limit is 150 Da, determining kinetic rate constants in the range of k_ass 10³ - 10⁶M^-1 s^-1 and         k_diss 10^-1 - 10^-5 s^-1. The flow rate can be chosen between 1 to 100 µl / min in steps of 1 µl. By the use of a recovery block a collection of specifically bound material from the surface is possible.

The Biacore 2000 instrument is best suited for antibody characterization and other protein-protein or protein-peptide interactions. Due to the high flexibility in assay design Biaffin uses two Biacore 2000 instruments.