Antibody Characterisation

Classical methods for the kinetic characterisation of antigen - antibody interactions like ELISA only provide steady state data at one defined time point not considering the rate of complex formation and complex dissociation.
BIA technology has been proven to be an excellent method to kinetically analyse antibody interactions by monitoring the binding kinetics between antigens and antibodies in real time. 
The binding curves contain information about the association and dissociation rate, of the stoichiometry of binding and thus about the binding activity and quality of different factions within a protein solution. Furthermore, an assessment of binding behaviour regarding heterogeneity, biphasic binding and avidity effects can be provided.