Enzyme & Protein Activity Assays
follow up kinase reaction and kinase inhibition in real time
Biaffin provides in vitro kinase assays using the microfluidic mobility-shift system of PerkinElmer. The method utilizes the shift in the electrophoretic mobility of a peptide substrate upon an enzymatic conversion. Thus, due to a change in charge the fluorescently labeled substrate and product are separately detected by a laser. Since substrate phosphorylation can be monitored in real time a detailed kinetic characterization of enzyme activity is possible.
The assays will be performed on a LabChip® Desktop Profiler in „off-chip“ mode (384 well format).
Benefit from our experience and obtain accurate results even for enzymes with low specific activity at any ATP concentration.
Our service:
- characterization of enzyme kinetics
- kinase activity evaluation
- analysing the effect of kinase activators (e.g. cAMP)
- kinetic characterization (KM value for ATP and substrate, Vmax and kcat)
- design of inhibitor screening studys
- qualitative screen of our compound libary (60 kinase inhibitors) or of small molecules supplied by the customer
- IC50 determination of kinase inhibitors
- characterization of the inhibition mode (ATP competitive, substrate competitive, Ki determination, compound binding reversibility test)
- evaluation of substrate specificty
- selection of a suitable substrate for kinase reaction
We would be glad to provide you an individual offer. Please get in contact with our application specialist for more detailed informations.
Tel.: +49 (0) 561-804 4661 | Fax: +49 (0) 561-804 4665 | info@biaffin.de
