Luminescent based Assays

universal method for measuring enzyme activity by ATP consumption

We offer service on kinase activity studies using the Promega Kinase Glo reagent for coupling kinase activity to luminescent reaction catalyzed by luciferase. The bioluminescence is inversely correlated to the depleted ATP in the solution upon kinase reaction. Since the assay procedure does no require any labeling of kinase assay components the method can be used for any kinase / substrate combination. In order to enable highest sensitivity kinase activity is measured at substrate K_m. The assay will be performed in 96 well plates in endpoint mode.

Our service:

determination of kinase activity at substrate K_m
qualitytive screen of kinase inhibitors
IC₅₀ determination of kinase inhibitors

We would be glad to provide you an individual offer. Please get in contact with our application specialist for more detailed informations.

Biaffin provides bioluminescence ATP determination kits ideally suited for sensitive quantification of ATP in various applications such as ATP detection in biological samples or monitoring of ATP dependent enzyme assays. The timestable assay is optimized for high throughput screening with nearly constant luminescence signals over a period of up to four hours. The sensitive assay can detect even nanomolar concentrations of ATP.

For purchasing our bioluminescent assay kits please visit our distribution platform www.proteinkinase.de.