BIA-MS enables identification of unknown binding partners

BIA-technology is ideally suited as a micro-affinity purification platform allowing on-line detection of binding events and direct quantification of bound material on sensor surfaces. Kinetic parameters may be obtained as additional information. Provided that a binding partner has been captured on the sensor chip it can be recovered by a gentle elution for further downstream analyses like mass spectrometry or western blot analysis.

In comparison to classical chromatographic methods biosensor systems allow monitoring and thus reducing unspecific binding, are compatible with small volume recovery and are readily automated. The identification of unknown ligands is of particular interest for revealing function of orphan receptors, for the detection of cellular interaction networks and for testing the biocompatibility of novel surface coatings.

Biaffin offers contract research for the identification of unknown binding partners using BIA technology in combination with a subsequent identification of these samples using mass spectrometry performed in a qualified cooperating lab. Please ask for an individual quote that meets your requirements.

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