Biaffin Protein/protein interactions

Biaffin - Service für Biomolekulare Interaktionsanalyse

1. QUALITATIVE Interaction Analysis :

Determine qualitatively the interaction of given binding partners for estimation of the K_D-value and of the expected kinetic of the interaction
Optimise buffer conditions to reduce unspecific binding
Determine the influence of pH, ionic strength and ligands on the given interaction
Optimise regeneration conditions including tests of functionality of the immobilised ligand after regeneration
Investigate mass transfer effects

2. QUANTITATIVE Interaction Analysis :

Determine concentration series of analytes, the basis for determining association rate constants
Calculate association and dissociation rate constants (kass, kdiss) by determining a row of concentrations
Evaluation of binding data including blank subtraction
Calculation of K_D-values using different algorithms depending on the binding kinetics
Determination of the formation of multiple protein complexes

Four parallel flow cells permit simultaneous interaction analysis of several interaction partners (e.g. four isoforms, deletion mutants or modifications of the same protein as shown below in the figure).